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KMID : 1098420070150010062
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 1 p.62 ~ p.66
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Plant Regeneration via Organogenesis from Leaf Explant Culture of Taraxacum coreanum Nakai
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Lee Hyun-Hwa
Kim Young-Soon
Park Hyeon-Yong
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Abstract
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Plant Regeneration via organogenesis from leaf disk of Korean dandelion was investigated. Leaf disk cultured on MS medium with various combinations of BA (0-4 mg/L) and 2,4-D (0-1 mg/L). Shoot regeneration from leaf explant was observed after 3 weeks of culture. The highest shoot regeneration frequency from leaf disk was obtained with 2 mg/L BA. To analyze the effect of leaf age along shoot formation, we measured number of shoots per explant, shooting rate, fresh and dry weight of leaf explant. The highest number of shoots (11.5) per explant were obtained leaf from 7 weeks old plantlets after seed germination. The regenerated shoots were transferred in 1/2 MS medium with 0.5 mg/L NAA for root formation. Regeneratied plantlets thought organogenesis were growing to whole plants in the pots with acclimation.
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KEYWORD
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Taraxacum coreanum Nakai., organogenesis, plantlet regeneration
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